💪 TB-500 Dosage Calculator
Choose your protocol phase (loading or maintenance), dose, vial size, and BAC water — draw volume and dosing schedule computed instantly for every injection.
TB-500 Reconstitution & Dose Calculator
TB-500 is a synthetic analogue of Thymosin Beta-4, a naturally occurring peptide concentrated in wound fluid and regenerating tissue. It promotes systemic cell migration and proliferation, accelerates actin polymerisation for wound closure, reduces inflammation, and has demonstrated significant effects on muscle, tendon, ligament, and cardiac tissue repair in preclinical and human research. Unlike BPC-157's local action, TB-500 distributes systemically — making it ideal for multi-site injuries or chronic systemic inflammation. It is supplied as lyophilised powder and reconstituted with bacteriostatic water for subcutaneous or intramuscular injection.
Select your protocol phase, weekly dose, vial size, and BAC water volume. The calculator returns concentration, per-dose volume, syringe units, and doses per vial.
You have a 5 mg TB-500 vial, add 1 mL BAC water, and inject 2.5 mg (2,500 mcg) on a 0.5 mL syringe.
- Concentration = (5 × 1,000) ÷ 1 = 5,000 mcg/mL
- Volume = 2,500 ÷ 5,000 = 0.5 mL
- U-100 draw = 0.5 × 100 = pull to the 50-unit mark
- Doses per vial = floor(5,000 ÷ 2,500) = 2 doses
Select your protocol phase
Dose per injection
TB-500 vial size
Bacteriostatic water added
Syringe volume
Select your inputs above to generate your draw guide.
Reference draw uses 5 mg vial + 1 mL BAC water = 5,000 mcg/mL. SC or IM injection.
| Phase | Duration | Dose | Frequency | Weekly Total | Draw (5mg/1mL) | 5mg Vials/Week |
|---|---|---|---|---|---|---|
| Loading | Wks 1–6 | 2.5 mg | Twice weekly | 5 mg | 50 units U-100 | 1 |
| Loading (high) | Wks 1–4 | 4 mg | Twice weekly | 8 mg | 80 units U-100 | 2 |
| Maintenance | Wk 7+ | 2 mg | Once weekly | 2 mg | 40 units U-100 | 1 (2–3 wks) |
| Low maintenance | Wk 7+ | 1 mg | Once weekly | 1 mg | 20 units U-100 | 1 (5 wks) |
TB-500 Calculator — Frequently Asked Questions
What is the difference between TB-500 and Thymosin Beta-4?
Thymosin Beta-4 (Tβ4) is a naturally occurring 43-amino acid peptide found throughout the body, with high concentrations in platelets, wound fluid, and regenerating tissue. TB-500 is a synthetic 17-amino acid fragment of Tβ4 corresponding to its most biologically active region — the actin-binding domain (amino acids 17–23). TB-500 retains the key regenerative properties of full-length Tβ4 (cell migration, anti-inflammation, angiogenesis) at a lower molecular weight, making it more practical for research use. Most published research on TB-500 shows results consistent with full Tβ4 data.
Why is TB-500 dosed twice weekly during loading but once weekly for maintenance?
TB-500 has a longer half-life than BPC-157 (~24–48 hours), which makes once-weekly maintenance dosing pharmacologically reasonable. The twice-weekly loading protocol is designed to rapidly saturate tissue receptors and achieve therapeutic tissue concentrations faster — similar to loading protocols used for creatine or some antibiotics. Once tissue levels are established (typically 4–6 weeks), once-weekly maintenance sustains the regenerative environment without unnecessary peptide expenditure. Some researchers use once-weekly dosing throughout, particularly for lower-dose protocols.
Can TB-500 and BPC-157 be injected at the same time?
Yes, they can be administered in the same session — either as separate injections at adjacent sites or occasionally mixed in the same syringe (though mixing peptides is generally not recommended as stability is unverified without testing). Using separate syringes ensures accurate dosing and avoids any potential interaction between compounds in solution. Injecting at the same site is possible but two nearby SC sites are cleaner practice. The combination stack (typically 500 mcg BPC-157 daily + 2 mg TB-500 2x/week) is one of the most discussed recovery protocols in peptide research.